Alternative Method for Direct DNA Probe Labeling and Detection Using the Checkerboard Hybridization Format
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چکیده
منابع مشابه
Alternative Method for Direct DNA Probe Labeling and Detection Using the Checkerboard Hybridization Format
Molecular diagnostic methods using genetic material probes have been employed in the health care field for the detection and quantitation of several species of microorganisms (2, 5). These methods are faster and more suitable than traditional culture methods. In addition, the possibility of the inclusion of difficult-to-culture, uncultivable, or uncharacterized species in the set of target spec...
متن کاملAlternative Method for Direct DNA Probe Labeling and Detection Using the Checkerboard Hybridization Format
Molecular diagnostic methods using genetic material probes have been employed in the health care field for the detection and quantitation of several species of microorganisms (2, 5). These methods are faster and more suitable than traditional culture methods. In addition, the possibility of the inclusion of difficult-to-culture, uncultivable, or uncharacterized species in the set of target spec...
متن کاملAlternative method for direct DNA probe labeling and detection using the checkerboard hybridization format.
Molecular diagnostic methods using genetic material probes have been employed in the health care field for the detection and quantitation of several species of microorganisms (2, 5). These methods are faster and more suitable than traditional culture methods. In addition, the possibility of the inclusion of difficult-to-culture, uncultivable, or uncharacterized species in the set of target spec...
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Comparative genomic hybridization (CGH) represents a powerful method for screening the entire genome of solid tumors for chromosomal imbalances. Particularly it enabled the molecular cytogenetic analysis of archival, formalin-fixed, paraffin-embedded (FFPE) tissue. A well-known dilemma, however, is the poor DNA quality of this material with fragment sizes below 1000 bp. Nick translation, the co...
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Methods for non-radioactive in situ hybridization are rapidly progressing in refinement and application. In this overview, we present our experiences with the use of synthetic oligodeoxynucleotides, labeled with biotin- or digoxigenin-tagged nucleotides by the terminal transferase method.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00390-10